Glutathione-s-transferase is a minor allergen in birch pollen because of restricted release from hydrated pollen grains
نویسندگان
چکیده
Methods bGST was expressed in Escherichia coli, purified and characterized by mass spectrometry. BALB/c mice were immunized with bGST or Bet v 1. Antibody and T cell responses were assessed. 217 sera from birch pollen-allergic patients were tested for IgE-reactivity to bGST by ELISA. The allergenicity of bGST was evaluated with IgE-loaded rat basophil leukaemia cells (RBL) expressing the a-chain of the human receptor FcεRI. Cross-reactivity of IgE between bGST and GST from house dust mite, Der p 8, was assessed with murine and human sera in ELISA. The release kinetics of bGST and Bet v 1 from birch pollen upon hydration were studied by immunoblotting.
منابع مشابه
Session 1 - Allergens : Production , Purification , Structure Analysis
O1 Glutathione-s-transferase is a minor allergen in birch pollen because of restricted release from hydrated pollen grains Stephan Deifl, Christian Zwicker, Eva Vejvar, Claudia Kitzmüller, Gabriele Gadermaier, Birgit Nagl, Susanne Vrtala, Gerhard Zlabinger, Peter Briza, Fatima Ferreira, Barbara Bohle Medical University of Vienna, Department of Pathophysiology and Allergy Research, Vienna, Austr...
متن کاملGlutathione-S-Transferase: A Minor Allergen in Birch Pollen due to Limited Release from Hydrated Pollen
BACKGROUND Recently, a protein homologous to glutathione-S-transferases (GST) was detected in prominent amounts in birch pollen by proteomic profiling. As members of the GST family are relevant allergens in mites, cockroach and fungi we investigated the allergenic relevance of GST from birch (bGST). METHODOLOGY bGST was expressed in Escherichia coli, purified and characterized by mass spectro...
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عنوان ژورنال:
دوره 4 شماره
صفحات -
تاریخ انتشار 2014